Fig. 2: Generation and characterization of GRP78-CAR T cells. | Nature Communications

Fig. 2: Generation and characterization of GRP78-CAR T cells.

From: CAR T cells redirected to cell surface GRP78 display robust anti-acute myeloid leukemia activity and do not target hematopoietic progenitor cells

Fig. 2

a Scheme of retroviral vectors encoding GRP78.1x-, GRP78.2x-, and GRP78.3x-CARs (‘Pep’ stands for peptide). b Transduction efficiency of GRP78-CAR constructs probing for tCD19 using flow cytometry on days 5–7 after retroviral transduction (control CAR: FRP5-CAR) (N = 3, 1x vs 2x p = 0.0008, 1x vs 3x p < 0.0001, 2x vs 3x p = 0.6131, two-sided T-test for pairwise comparisons was used). c Cell viability of CAR T cells measured from day 2 to day 9 post transduction using trypan blue (left panel, N = 3, p = ns, two-sided T-test for pairwise comparisons was used). Expansion of NT, Control CAR or GRP78 peptide CAR T cells measured for 7 days after transduction (right panel, N = 3, p = ns, two-sided T-test for pairwise comparisons was used). d Flow cytometric analysis of immunophenotype of GRP78 CAR transduced cells on day 7 post transduction (EM: CCR7−, CD45RA−; CM: CCR7+, CD45RA−; Naive-like: CCR7+CD45RA+; TEMRA: CCR7-, CD45RA+) (N = 3, T-test was used with pairwise comparisons, p < 0.05). The data in Fig. 2 are presented as mean values ± SD. Source data are provided as a Source data file.

Back to article page