Fig. 1: TAZ knockout in muscle decreases mitochondrial mass, respiration, and exercise ability.
From: TAZ links exercise to mitochondrial biogenesis via mitochondrial transcription factor A
a Images of the gastrocnemius muscle of wild-type (WT) or muscle-specific TAZ knockout (mKO) mice were acquired using a transmission electron microscope; the mitochondrial area or number of mitochondria per μm2 was counted and calculated using Image J. Scale bar = 0.5 μm, n = 3 for each genotype (Mitochondrial area; *p = 0.022, mitochondrial number; *p = 0.0104). b Proteins of the gastrocnemius muscle of WT and mKO mice were analysed via immunoblotting for the detection of mitochondrial marker proteins. Alpha-tubulin was used as the loading control. Representative data was shown and the experiment was performed twice with similar results. c The respiratory and metabolic parameters of WT and mKO mice were assessed via indirect calorimetry tests. Data are shown as average values; n = 7 for each group (VO2; *p = 0.0353 for light, **p = 0.0089 for dark, VCO2; *p = 0.0134, Energy expenditure; *p = 0.0137). d The activity of mice under normal physiological conditions was analysed using a metabolic cage; n = 7 for each group (Rearing; *p = 0.036, Activity; *p = 0.0267). e Food and water consumption of mice described in (a) were monitored with a metabolic cage; n = 7 for each group (Food intake; *p = 0.014). f The exercise ability of WT and mKO mice was analysed using the rotarod test; n = 8 for WT mice and n = 10 for mKO mice (1 day; **p = 0.00789, 2 day; ***p = 0.00158. 3 day; ***p = 0.00022). g Endurance test of WT and mKO mice was conducted using a rodent treadmill; n = 10 for WT mice and n = 12 for mKO mice (***p = 0.0017). Eight to ten-week-old mice were used. Data are presented as mean ± SEM for (a, c, d–g). Statistical significance was analysed using two-sided t-test for (a, c, e–g) and one-sided t-test for (d). Source data are provided as a Source data file.
