Fig. 5: Pre-treatment with 10 and 20 mg/kg unmodified 10E8v4 and 10E8v4 EFTAE decreases post-acute plasma viremia in macaques.
a Longitudinal or b TOC plasma bNAb concentrations following 10 (dashed lines) or 20 (solid lines) mg/kg 10E8v4 subcutaneously delivered 3 days prior to challenge (day –3). The average half-life (t1/2) ± se calculated from the n = 4 animals in each group (Supplementary Fig. 2) is shown in the right panel of (a). c Plasma neutralization titers against SHIVSF162P3 pseudovirus at TOC. d bNAb concentrations in biopsies collected from the rectal mucosa 5 days after challenge (8 days post delivery). e Individual and f average ± se longitudinal plasma viremia of macaques in each mAb treatment group. A dashed line at 106 vRNA copies/ml is presented in (e) as a visual reference. Average g peak or h post-acute PVL, defined as from day 28–63 with a table insert showing fold-difference compared to the control group. Boxes in box and whisker plots extend from 25 to 75 percentiles with a line at median and whiskers extending to min–max values. Statistical comparisons were performed using a one-way (b, c, d, g) or two-way ANOVA (h) followed by Tukey’s post-hoc test between groups. All <0.05 adjusted p values are shown. Boxes in box and whisker plots extend from 25 to 75 percentiles with a line at median and whiskers extending to min–max values. Statistical comparisons were performed using a one-way ANOVA (b, c) followed by Tukey’s post-hoc comparison between groups and Dunnett’s post-hoc test for comparison to control group (g), two-way repeated-measures ANOVA (h) followed by Tukey’s post-hoc comparison. Data and analysis are derived from n = 4 animals per group with symbols denoting individual macaques as indicated in (e). Viral loads are presented and analyzed using log10 transformed data. Group colors are consistent throughout the manuscript. Statistical significance was determined at the significant alpha level of 0.05 and performed in GraphPad Prism 9. Data in b–d, g, and h are representative of at least two independent experiments. Source data are provided in the Source Data file associated with this manuscript. TOC time of challenge, PVL plasma viral load.
