Fig. 4: BacNav expression enhances cardiomyocyte excitability in vitro.
From: Engineered bacterial voltage-gated sodium channel platform for cardiac gene therapy
a, b Transduction of NRVMs with MHCK7-GFP (“GFP”) or MHCK7-h2SheP-2A-GFP (“h2SheP”) lentivirus did not affect mRNA expression of SCN5A gene shown normalized to B2M housekeeping gene (a, n = 11) or Nav1.5 current density (b, n = 5 for no virus group; n = 6 for GFP group; n = 10 for h2SheP group). c Representative current responses to a voltage step from −80 mV (holding) to −20 mV demonstrating slower kinetics of h2SheP than Nav1.5 current. d, e Representative h2SheP current responses (d) and peak INa–V curve (e, n = 7) in NRVMs transduced with MHCK7-h2SheP lentivirus. Only traces corresponding to stepping voltages at 0–50 mV are shown in d. f–j Representative AP traces measured via intracellular recording with inset showing AP upstrokes and corresponding quantifications of maximum AP upstroke velocity (g), APA (h), APD80 (i), and RMP (j) in No virus (n = 32), GFP (n = 10), and h2SheP (n = 19) groups. ****P < 0.0001 versus h2SheP group in g and h. Electrophysiological recordings were performed at 25 °C in b, c and at 37 °C elsewhere. Error bars indicate s.e.m; statistical significance was determined by one-way ANOVA, followed by Tukey’s post-hoc test to calculate P-values. Source data are provided as a Source Data file.
