Fig. 4: Binding of GFP-AtlAEfs to GAS sacculi.
From: PplD is a de-N-acetylase of the cell wall linkage unit of streptococcal rhamnopolysaccharides
Intact sacculi (untreated), sacculi subjected to mild acid hydrolysis (mild acid), or deamination with HONO (HONO) were incubated with GFP-AtlAEfs, and examined by fluorescence microscopy (GFP-AtlAEfs, top panels) and differential interference contrast (DIC, middle panels). An overlay of fluorescence and DIC (merge) is shown in the bottom panels. The experiments were performed independently three times and yielded the same results. Representative image from one experiment is shown. Scale bar is 1 µm.
