Fig. 1: LPR2-dependent growth inhibition of primary roots in NH4+ medium.
From: Phloem iron remodels root development in response to ammonium as the major nitrogen source
a Phenotype comparison of Col-0 and the isas, lpr2-1, lpr1-1, and lpr1lpr2 mutants. b Elongation of primary roots. c Phenotype comparison of Col-0, lpr2-1, and complementation lines (COM #7 and COM #15). d Number of meristematic cells. e Number of elongation cells. f Length of the first differentiated cell. g Length of the root growth zone (elongation plus meristem zone). b, d-g Data shown are mean ± SD. P values < 0.05 indicate significant differences (two-way ANOVA with post-hoc Tukey HSD test; n = number of seedlings). Four-day-old seedlings of the indicated genotypes were transferred to NO3− or NH4+ medium with 100 µM Fe and analyzed 4 days after seedling transfer. Each experiment was repeated independently at least three times with similar results, and a representative experiment is shown.
