Fig. 4: Distribution of LPR2 in roots and its response to NH4+.
From: Phloem iron remodels root development in response to ammonium as the major nitrogen source
a pLPR2::LPR2-YFP expression in primary roots of complementation line COM#7. Roots were counterstained with propidium iodide (purple fluorescence) and analyzed for YFP fluorescence (green). b Radial section (top) and close-up view (bottom) of pLPR2::LPR2-YFP expression in a root of a COM#7 seedling grown in Fesuff NH4+ medium. c LPR2 expression in Col-0 roots. Relative expression levels were normalized to the geometric mean of expression of UBQ10 and EF1α. d, e Representative gels and relative protein levels in Col-0 roots. Relative LPR2 levels were estimated from the ratio of the signal intensity of LPR2 to that of actin from the same sample. c, e Data shown are mean ± SD of three biological replicates. P values < 0.05 indicate significant differences (two-way ANOVA with post-hoc Tukey HSD test). Four-day-old seedlings of the indicated genotypes were transferred to NO3− or NH4+ medium with Fesuff (100 µM) or Felow (10 µM) and analyzed 4 days after seedling transfer. Each experiment was repeated independently three times with similar results, and a representative experiment is shown.
