Fig. 1: Discovery of the lap gene cluster. | Nature Communications

Fig. 1: Discovery of the lap gene cluster.

From: Lapcin, a potent dual topoisomerase I/II inhibitor discovered by soil metagenome guided total chemical synthesis

Fig. 1

a Overview of the PABA-specific A-domain guided discovery of the lapcin (lap) BGC from the soil metagenome. i DNA extracted from soil was used to construct metagenomic libraries. ii NPSTs generated from arrayed metagenomic libraries and iii the resulting NPSTs were searched for PABA-specific A-domains based on a signature sequence derived from known PABA A-domains. iv Phylogenetic analysis of predicted PABA NPSTs was used to identify sequences that arose from a new family of BGCs. v Clones containing NRPS BGCs of interest were recovered from the arrayed library subpools and fully sequenced to reveal BGCs that encode PABA-based natural products. b The lap BGC, which is shown here, was recovered from an archived soil metagenome library using this process. olive, resistance gene; red, NRPS biosynthesis; green, PABA tailoring; blue, thiazole formation; pink, PABA core.

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