Fig. 1: Viruses derived Ades efficiently inhibit A3-CBEs.
From: Inhibition of base editors with anti-deaminases derived from viruses
a Schematic representation of anti-CRISPR and anti-deaminase. b Schematic representation of three A3-CBE architectures. nCas9, D10A. c, d Base editing of A3A-, A3B- and A3G-CBE in the presence or absence of the seven Ades at the EMX1-1 (c) and FNACF (d) sites. Plasmids expressing CBE, sgRNA, and each Ade (1:1:1) were cotransfected into HEK293T cells. e–g Inhibition of A3A-CBE (e), A3B-CBE (f) and A3G-CBE (g) with different doses of Ades at the EMX1-1 site. The ratio of Ade:CBE ranging from 1:4 to 6:1. Values and error bars reflect the mean ± s.e.m. and n = 3 biologically independent experiments. All p values were calculated by two-sided t tests. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.
