Fig. 5: Evaluation of Cas9-dependent and Cas9-independent OT editing.
From: Inhibition of base editors with anti-deaminases derived from viruses
The principle of the assay is illustrated on the left, and the results are shown as bar graphs on the right. a On-target editing frequencies of A3A and A3Bctd-CBE in the presence or absence of AcrIIC1 (as a negative control), AcrIIA5, Ade1 and Ade2 at the EMX1-1 site. b Cas9-dependent OT editing frequencies of A3A and A3Bctd-CBE in the presence or absence of AcrIIC1 (the negative control), AcrIIA5, Ade1 and Ade2 at the EMX1-1 OT1 and OT2 sites. c Cas9-independent OT editing frequencies of A3A and A3Bctd-CBE in the presence or absence of AcrIIC1 (the negative control), AcrIIA5, Ade1 and Ade2 at Sa site5, site6, site14 and site17. The dSaCas9 together with a gRNA was used to induce a stable ssDNA region (orthogonal R loop) at a specific locus, thus artificially magnifying Cas9-independent deamination. Values and error bars reflect the mean ± s.e.m. and n = 3 biologically independent experiments. All p values were calculated by two-sided t tests. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.
