Fig. 6: Hepatocyte-specific base editing by A3Bctd-CBE in cultured cells.
From: Inhibition of base editors with anti-deaminases derived from viruses
a Schematic of the CBE-ON switch. The miR-122 binding sites were inserted into the 3′UTR of an Ade-encoding transgene. Upon delivery, Ade expression is selectively knocked down within the target cells, permitting CBE activity. In OFF-target cells lacking the miR-122 signature, the Ade protein is translated and inhibits A3Bctd-CBE. b, c HEK293T and Huh7 cells were transfected with plasmids encoding A3Bctd-CBE, sgRNA and Ade1 with or without 3xmiR-122BS at the EMX1-1 site (b) and FANCF site (c). Values and error bars reflect the mean ± s.e.m. and n = 3 biologically independent experiments. All p values were calculated by two-sided t tests. **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.
