Fig. 5: ROCK inhibition selectively antagonizes in vivo growth of TEAD/YAP driven human tumors.
From: ROCK1 mechano-signaling dependency of human malignancies driven by TEAD/YAP activation
a–c Tumor cell growth following orthotopic inoculation in immunocompromised mice of MDA-MB-468, MDA-MB-231 (a) or HCC1395 cells (b) followed by daily dosing of H-1152 (25 mg/kg) after tumors reached a volume of 100 mm3. n = 5 mice per group were used. p values are indicated in the figure calculated using two tailed t-test for n = 5 biologically independent mice per group. Body weight of each mouse at the beginning and at the end of the treatment is also shown (c). d Immunostaining of tumor sections from (a, b) with antibodies directed against pCofilin as described in the Methods. Scale bar: 100 µM. n = 5 biologically independent mice per group. e Model depicting inhibition of TEAD/YAP transcription in physiological conditions, activated by p53 DNA contact mutations or Hippo pathway alterations by ROCK inhibitiors.*p < 0.05, **p < 0.01. Source data with actual calculated p values are provided as Source data file.
