Fig. 1: Rhamnolipid production differs between liquid culture and surface-attached P. aeruginosa.
From: Spatial-temporal dynamics of a microbial cooperative behavior resistant to cheating
a Cartoon depictions of liquid and spatially-structured environments used in this study. b Optical density timeseries describing P. aeruginosa growth in liquid culture. [Blue] Biomass growth without exogenous quorum signals. [Purple] Biomass growth with exogenous quorum signals. c DsRed fluorescent timeseries generated from a custom-built imager (Supplementary Fig. 1) and custom software (Supplementary Fig. 3) describing P. aeruginosa growth in colony forming units (CFU). [Blue] Biomass growth without exogenous quorum signals [Purple] Biomass growth with exogenous quorum signals added to the plate media. [Inset] Example plate showing colonies at 48 h. Scale bar 1 cm. d Promoter activity \(\left[\frac{{dGFP}}{{dt}}\cdot \frac{1}{{{OD}}_{600}}\right]\) of PrhlAB with respect to culture growth rate \(\left[\frac{d{{OD}}_{600}}{{dt}}\cdot \frac{1}{{{OD}}_{600}}\right]\). [Blue] without exogenous quorum signals [Purple] with exogenous quorum signals. e Promoter activity \(\left[\frac{{dGFP}}{{dt}}\cdot \frac{1}{{DsRed}}\right]\) of PrhlAB with respect to CFU growth rate \(\left[\frac{{dDsRed}}{{dt}}\cdot \frac{1}{{DsRed}}\right]\). [Blue] without exogenous quorum signals [Purple] with exogenous quorum signals provided in the plate media.
