Fig. 4: LPO inputs to the LHb highly express MOR mRNA.
From: A diencephalic circuit in rats for opioid analgesia but not positive reinforcement
a Schematic of unilateral iontophoretic deposition of the retrograde tracer FG into the LHb. b FG injection sites in LHb (n = 3 rats). c (Left and middle) Representative brightfield images of FG-positive/MOR-positive somas (orange arrowhead) and FG-positive/MOR-negative somas (blue arrowhead) in the LPO. Scale bar = 20 µm. (Right) Darkfield image of in situ hybridization localization for MOR mRNA (OPRM1). d Number of MOR-positive/FG-positive (orange) and MOR-negative/FG-positive (blue) cells in various basal forebrain regions (data are indicated as bar graphs with mean ± SEM, and circles as individual animals). e Donut plot depicting proportions of total MOR-positive inputs to the LHb from various basal forebrain regions. f Locations of FG-positive/MOR-positive (orange) and FG-positive/MOR-negative (blue) somata throughout basal forebrain. Co-labeled cells are separated from the singly labeled population for display purposes.
