Fig. 2: LC–MS/MS enables the identification of chemically defined dietary wheat peptides in human urine. | Nature Communications

Fig. 2: LC–MS/MS enables the identification of chemically defined dietary wheat peptides in human urine.

From: An efficient urine peptidomics workflow identifies chemically defined dietary gluten peptides from patients with celiac disease

Fig. 2

a Pilot study design. b Number of wheat-derived peptide sequences detected in the urine of 4 healthy participants (4 males, 24-28 years of age) after a 24-h gluten-free diet and after a dietary challenge with wheat gluten. Horizontal line represents median. c Simplified study design. d Number of wheat-derived peptide sequences detected in 8 healthy participants (5 males, 3 females, 24-28 years of age) after an overnight fast and after a dietary challenge with wheat gluten. Horizontal line represents median. In all LC–MS/MS experiments, peptide identifications were controlled at a false discovery rate of 1% using the PEAKS software decoy-fusion approach59. Source data are provided as a Source Data file.

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