Table 1 Detailed characterization of isolated murine BG505.MD39-specific clones in terms of VH, VJ, VD, VK, VK, VJ gene usage, HCDR3 and LCDR3 lengths.
Antibody clone | HV gene | HD gene | HJ gene | CDRH3 length | HV %identity | KV gene | KJ gene | CDRK3 length | KV %identity | Barcode percentage |
|---|---|---|---|---|---|---|---|---|---|---|
C3 | 3-2*02 | 2-4*01 | 4*01 | 12 | 100.00 | 4-91*01 | 2*01 | 9 | 100.00 | 12.5 |
C4 | 14-1*02 | 1-1*01 | 2*01 | 9 | 100.00 | 4-70*01 | 4*01 | 8 | 100.00 | 9.375 |
C5 | 6-6*02 | 1-2*01 | 4*01 | 13 | 95.92 | 3-2*01 | 2*01 | 9 | 96.91 | 9.375 |
C6 | 6-6*02 | 1-2*01 | 4*01 | 13 | 95.92 | 3-2*01 | 2*01 | 9 | 96.91 | 6.25 |
C10 | 5-6-4*01 | 2-2*01 | 2*01 | 17 | 88.54 | 3-7*01 | 1*01 1*02 | 9 | 91.41 | 3.125 |
C11 | 6-6*02 | 1-2*01 | 2*01 | 10 | 98.98 | 5-43*01 | 2*01 | 10 | 100.00 | 3.125 |
C13 | 11-2*02 | 2-3*01 | 1*01 | 12 | 100.00 | 14-126*01 | 5*01 | 9 | 100.00 | 3.125 |
C16 | 14-4*02 | 1-2*01 | 2*01 | 13 | 100.00 | 13-84*01 | 1*01 | 8 | 100.00 | 3.125 |
C18 | 1-61*01 | 2-4*01 | 3*01 | 12 | 96.53 | 4-50*01 | 5*01 | 9 | 100.00 | 3.125 |
C19 | 2-9*02 | 3-1*01 | 4*01 | 9 | 100.00 | 4-61*01 3-4*01 | 5*01 1*01 | 9 | 100.00 | 3.125 |
C20 | 1-69*02 1S126*01 | — | 2*01 | 8 | 94.44 | 13-84*01 | 5*01 | 9 | 100.00 | 3.125 |
C22 | 6-6*02 | 1-2*01 | 4*01 | 13 | 92.86 | 3-2*01 | 2*01 | 9 | 96.56 | 3.125 |
C23 | 4-1*02 | 2-3*01 | 3*01 | 11 | 95.83 | 4-86*01 | 5*01 | 9 | 97.46 | 3.125 |
C24 | 6-6*02 | 1-2*01 | 4*01 | 13 | 92.86 | 3-2*01 | 2*01 | 9 | 98.28 | 3.125 |
C25 | 9-1*02 | 2-4*01 | 4*01 | 12 | 100.00 | 3-4*01 | 2*01 | 9 | 100.00 | 3.125 |
C26 | 14-3*02 | 1-2*01 | 2*01 | 9 | 100.00 | 10-96*01 | 2*01 | 9 | 100.00 | 3.125 |
C27 | 1-62-2*01 1-71*01 | 2-1*01 | 3*01 | 14 | 99.31 | 4-86*01 | 1*01 | 9 | 100.00 | 3.125 |
C28 | 2-2*02 | 2-10*01 | 3*01 | 13 | 100.00 | 1-110*01 | 2*01 | 9 | 100.00 | 3.125 |
C30 | 1-18*01 1-22*01 | 3-1*01 | 4*01 | 11 | 96.53 | 16-104*01 | 2*01 | 9 | 99.64 | 3.125 |
C32 | 9-3*02 9-3*03 | 1-1*01 | 3*01 | 13 | 100.00 | 4-50*01 | 5*01 | 9 | 100.00 | 3.125 |
C33 | 6-6*02 | 1-2*01 | 4*01 | 13 | 97.28 | 3-2*01 | 2*01 | 9 | 100.00 | 3.125 |
C34 | 5-9-1*01 | 2-10*02 | 2*01 | 10 | 100.00 | 19-93*01 | 2*01 | 8 | 100.00 | 3.125 |
C35 | 1S81*02 | — | 2*01 | 6 | 96.53 | 8-21*01 | 5*01 | 8 | 100.00 | 3.125 |
C37 | 6-6*02 | 1-2*01 | 4*01 | 13 | 95.92 | 3-2*01 | 2*01 | 9 | 97.94 | 3.125 |
