Fig. 4: Screening of CloA orthologues.
From: Reconstituting the complete biosynthesis of D-lysergic acid in yeast
a The putative oxidation and isomerization reactions catalyzed by CloA. b LC-MS/MS chromatograms of the products showing the ion transition of 269 → 223 m/z, produced by the CloA orthologs. Chromatograms appear in the order (bottom to top): pYES2-CT, DLA standard, C.glo (X90), M.rob (392), E.coe (253), C.fus, C.pur (20.1), B.cin, M.acr (SJ7), C.glo (ET3), P.ipo, M.acr (AT5), C.pas, C.pur, N.lol, E.coe (XN6). c Relative amounts of agroclavine consumed by the screened CloA orthologues. d Relative amounts of DLA produced by the various CloA orthologues. Data are presented as mean values + /− standard deviation. Error bars represent standard deviations calculated from three biological replicates. Source data are provided as a Source Data file.
