Fig. 5: Assembling the functional parts into a DLA-producing yeast strain.
From: Reconstituting the complete biosynthesis of D-lysergic acid in yeast
a Stepwise extension approach towards pathway construction in our engineered strains, enabling the use of each prior strain as a control for subsequent strains. b LC-MS/MS extracted ion chromatogram monitoring for the ion transition of [M + H]+ = 257 → 226 m/z, showing the production of chanoclavine-I in both AgcM1B and AgcM2B. c LC-MS/MS extracted ion chromatogram for the ion transition of [M + H]+ = 239 → 208 m/z, showing the production of agroclavine from AgcM33B. d LC-MS/MS extracted ion chromatogram showing the detected ion transition of [M + H]+ = 269 → 223 m/z, showing the production of DLA from DLAM33B. Peaks eluted at the time segments in blue correspond to the targeted compounds. Source data are provided as a Source Data file.
