Fig. 4: Pairwise enrichment. | Nature Communications

Fig. 4: Pairwise enrichment.

From: Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates

Fig. 4

The heatmaps show how the observed frequency of amino acid residues in two randomised positions (\({f}_{a,b}^{{obs}}\)) divided by the expected frequency in an ideally balanced library (\({f}_{a}^{{id}}\cdot {f}_{b}^{{id}}\)). A logarithmic scale is used to display both enrichment (red) and depletion (blue). Each panel represents one pair of randomised positions. The presence of large hydrophobic residues Leu/Ile at any one of positions 7a, 9 or 11 serves as an “anchor”, so that these residues appear in combination with non-preferred amino acids. n = 29,563 for each individual heatmap.

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