Fig. 3: Quantification of biomarkers in human biological fluids using biosensors based on circularly permutated version of LumiLuc.
From: Engineering and exploiting synthetic allostery of NanoLuc luciferase
a Quantification of α-amylase in samples of human saliva using solution assay based on cpLumiLuc biosensor (X-axis) and its comparison with and its comparison with the clinical α-amylase activity assay (Y-axis). b Quantification of HSA concentration in serum of human donors using cpLumiLuc HSA biosensor (X-axis) or clinical chemistry analyser (Y-axis). c Schematic representation of the BRET biosensor based on cpLumiLuc. d Emission scan of the solution of 1 nM of LumiLuc biosensor, 10 nM Rapamycin, 30 μM 8pyDTZ, e titration of 1 nM solution of LumiLuc biosensor and 30 μM 8pyDTZ with increasing concentrations of rapamycin. The emission was collected at 595–620 nm. f As in c but using the sample supplemented with 20% lysed human blood. Source data are provided as a Source Data file.
