Fig. 1: Reactivity of HIV-1 Env antigens with V2 monoclonal antibodies.
a A multiplex bead binding assay was used to determine the levels of reactivity of mAbs specific for different V2 epitopes with various HIV-1 antigens. The clade from which each antigen was derived is indicated by the subscripted letter and by color coding. As a control, V3 mAb 447-52D was used. Irrelevant mAbs and PBS were used as negative controls in each experiment (not shown). Data are shown as AUC values generated from titration curves for each mAb, provided in Supplementary Fig. S1. Strength of binding is color-coded as per the spectrum shown in the figure. Experiments were performed at least twice. b Statistical analyses comparing binding to cV2 peptides and V1V2-1FD6 proteins by V2p mAbs (CH58, CH59, CAP228_19F, CAP228_3D.1, and CAP228_16H) (p < 0.0001) (left graph) and V2i mAbs (830 A, 697-30D 2158, 1361) (p = 0.0006) (right graph) are shown along with a comparison of V2p mAbs (CH58, CH59, CAP228_19F, CAP228_3D.1, and CAP228_16H) vs cV2 peptides and V1V2-1FD6 proteins derived from HIVAE/92TH023 and HIVAE/A244 (p = 0.125) (center graph). Color of symbols show the clade/strain of the antigen used as per those used in Fig. 1a and Supplementary S2. Lines connect pairs of a given mAb with reagents from a given strain. For example, the connected red symbols in the left panel of Fig. 1b represent the reactivity of V2p mAb CH59 with cV2C/ZM109 and V1V2C/ZM109-1FD6. Statistical analyses were performed with the two-tailed Wilcoxon matched-pairs signed-rank test and are shown for: all HIV-1 strains used in Figure1b (left and right graphs) or for V2p mAbs reacting to the HIV-1 strain A244 (middle graph). Source data are provided as a Source Data file. AA amino acid, Ab antibody, Ags antigens, AUC area-under-the-curve, consC consensus C, cV2 cyclic V2, Env envelope, mAb monoclonal antibody.
