Fig. 4: Antibody binding, C1q binding, and ADCP responses of NHPs infected with SHIV (blue) (n = 20) or immunized with one of the three immunization regimens (red) (n = 25).

Each data point is derived from a biologically independent plasma specimen. Each panel shows results from a different assay: a multiplex bead Ab binding, b C1q binding, and c ADCP. Activities were measured using cV2 peptides (reactive with V2p Abs) or V1V2-1FD6 scaffold proteins (reactive with V2i Abs). Binding values in panel a are ΔMFI as shown in Fig. 3. Values in panels b and c represent area-under-the-curve (ΔAUC) and were calculated for C1q from two-fold plasma titrations ranging from 1:50–400, and for ADCP from five-fold dilutions ranging from 1:50–781,250 (Supplementary Fig. S6). ΔAUC values were calculated as the AUC from each NHP group 2 weeks after immunization or 11 (SHIV Tier 2) or 18 (SHIV Tier 1) weeks after infection from which the pre-bleed background response was subtracted. The mean of all animals within each group and the standard deviation are indicated. C1q titrations were performed once and ADCP experiments were performed at least twice. Source data are provided as a Source Data file.