Fig. 4: ENSA promotes tumor growth by activating STAT3. | Nature Communications

Fig. 4: ENSA promotes tumor growth by activating STAT3.

From: Copy number amplification of ENSA promotes the progression of triple-negative breast cancer via cholesterol biosynthesis

Fig. 4

a Candidate transcription factor (TF) prediction performed by GSEA of regulatory target gene sets. The top 6 TFs (P < 0.05) ranked by absolute normalized enrichment scores are shown. NES score and nominal P-value were given by GSEA software. b Western blotting images showing the protein levels of phosphorylated STAT3 (pSTAT3)-Tyr705, pSTAT3-Ser727 and total STAT3 after ENSA knockdown in BT549 and MDA-MB-231 cells. n = 3 independent experiments. c Western blotting images showing the protein levels of pSTAT3-Tyr705 and total STAT3 in BT549 and MDA-MB-231 cells ± ENSA knockdown and ± ENSA overexpression. n = 3 independent experiments. d In vitro growth curves of BT549 and MDA-MB-231 cells ± ENSA knockdown and ± STAT3 overexpression. n = 6. Data are presented as mean ± SD. Two-tailed two-way ANOVA tests. e Colony formation of BT549 and MDA-MB-231 cells ± ENSA knockdown and ± STAT3 overexpression. n = 3. Data are presented as mean ± SD. Two-tailed unpaired Student’s t tests. f In vivo growth curve of tumors (n = 6) generated by injecting MDA-MB-231 cells expressing control or ENSA shRNA and rescued by ENSA or STAT3 overexpression. n = 6 mice per group. Data are presented as mean ± SD. Two-tailed two-way ANOVA tests. g Tumor weight of MDA-MB-231 cells (n = 6) expressing control or ENSA shRNA rescued by ENSA or STAT3 overexpression. n = 6 mice per group. Data are presented as mean ± SD. Two-tailed unpaired Student’s t tests. h Immunohistochemical images of ENSA, pSTAT3-Tyr705, and cleaved caspase 3 in mammary fat pad xenograft models. Scale bar: 100 µm. Source data are provided as a Source Data file. NES normalized enrichment score.

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