Fig. 5: Tissue-specific RNAi of daf-16 or sbp-1. | Nature Communications

Fig. 5: Tissue-specific RNAi of daf-16 or sbp-1.

From: Histone H3K4me3 modification is a transgenerational epigenetic signal for lipid metabolism in Caenorhabditis elegans

Fig. 5

a Strains used for the tissue-specific gene knockdown. b–f Western blots (left panels) and quantification (right panels) of histone H3K4me3 modification in worms subjected to neuron-specific sbp-1 RNAi (b), muscle-specific sbp-1 RNAi (c), germline and intestine-specific sbp-1 RNAi (d), intestine-specific sbp-1 RNAi (e), germline-specific sbp-1 RNAi (f) (mean ± SD; n = 3 biologically independent samples; unpaired two-tailed Student’s t-test; ns, not significant). g–l quantification of ORO staining in animals subjected to muscle-specific daf-16 RNAi (n ≥ 31 per condition) (g), neuron-specific daf-16 RNAi (n ≥ 30 per condition) (h), intestine-specific daf-16 RNAi (n ≥ 30 per condition) (i), germline and intestine-specific daf-16 RNAi (n ≥ 30 per condition) (j), germline-specific daf-16 RNAi (n ≥ 31 per condition) (k) and without any RNAi exposure (n ≥ 30 per condition) (l). For g–l, graph data are presented mean ± SD; unpaired two-tailed Student’s t-test; ***p < 0.001, ns: no significance; scale bar = 150 μm. Source data are provided as a Source Data file.

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