Fig. 4: Batch normalization recapitulates bead normalization performance.
From: CytofIn enables integrated analysis of public mass cytometry datasets using generalized anchors
A Comparison of batch and bead normalized mean expression across 989 leukemia samples using MSFTB and BL normalizations. The differences between batch and bead normalized signals were quantified by the RMSD values. B Correlation analysis between batch and bead normalized expression of 36 consensus marker expressions from one selected patient sample. The R2 values normalized using MSFTB and BL normalizations were 0.999, and 0.996, respectively. C Comparison of 15 ALL subpopulation frequencies in 50 selected leukemia patient samples normalized by MSFTB and BL normalization functions compared to bead standardization (see Supplementary Fig. 10 for the complete quantification of 15 subpopulation frequencies normalized by each normalization method). D Mean expression of four markers: CD45, CD10, pS6, and pCreb in 15 subpopulations of 50 selected leukemia patient samples normalized using MSFTB, BL, or bead normalization (see Supplementary Fig. 11 for the complete quantification on the mean expression of four markers by each of the five normalization functions). Source data are provided as a Source Data file.
