Fig. 1: GLl3 binds to poised, accessible chromatin prior to HH signaling.
From: GLI transcriptional repression is inert prior to Hedgehog pathway activation
a Schematic for HH induction timeline during limb development. b Quantification of 21–25 S embryos with expression of Gli1 and Shh assayed by in situ hybridization. c Representative western blot (n = 3) of endogenous GLl3FLAG protein in the limb bud pre-(21–23 S) and post-HH signaling (32–35 S). d Venn diagram of all pre- and post-HH identified GLl3 CUT&RUN called peaks. e Percentage of E10.5 GLl3-bound regions also bound at E9.25, before HH signaling, for all E10.5 identified GBRs and E10.5 HH-responsive GBRs. f Venn diagram of HH-responsive GBRs enriched for poised enhancer marks and bound by GLl3 at E9.25. Poised enhancer modifications identified by H3K4me1 CUT&Tag (n = 3), H3K4me2 ChlP-seq (n = 2), ATAC-seq (n = 2). g Heatmap of GLl3 enrichment pre and post-HH signaling and enrichment of poised enhancer marks pre-HH signaling. Many regions lacking GLl3 also lack enrichment of poised marks. h Example of a HH-responsive GBR (orange shading) that is poised, accessible, and bound by GLl3 prior to HH signaling at a limb-specific Ptch1 enhancer26. i Example of a validated HH-responsive GBR GRE1, that regulates the GLl3 target Gremlin27, that is inaccessible, lacks H3K4me1 and GLl3 binding at E9.25. Scale bars = 1 kb. Source data are provided as a Source Data file.
