Fig. 1: The human mitochondrial transcriptome contains three 2′-O-methylated residues, modified by MRM1, MRM2 and MRM3. | Nature Communications

Fig. 1: The human mitochondrial transcriptome contains three 2′-O-methylated residues, modified by MRM1, MRM2 and MRM3.

From: A late-stage assembly checkpoint of the human mitochondrial ribosome large subunit

Fig. 1

a Determination of ribose methylation levels of known targets of MRM1 (G2815), MRM2 (U3039) and MRM3 (G3040). Experimental values and mean are shown. Statistical significance was assessed using two-tailed Student’s t test (∗: P ≤ 0.05; ∗∗: P ≤ 0.01; ∗∗∗: P ≤ 0.001; Cleavage protection score: G2815 Parental vs MRM1 KO P = 0.0138, G3039 Parental vs MRM2 KO P = 0.0122, G3039 Parental vs MRM3 KO P = 0.0122, G3040 Parental vs MRM3 KO P = 0.0003; RT stop score: G2815 Parental vs MRM1 KO P = 0.0236, G3040 Parental vs MRM3 KO P = 0.0235). b Mitochondrial transcriptome-wide evaluation of 2′-O-methylation in cells devoid of MRM1, MRM2 or MRM3 (n = 3 for each cell line). P values were determined by two-tailed Student’s t test. Source data are provided as a Source Data file.

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