Fig. 5: Structure of mtLSU assembly intermediates isolated in the absence of MRM2.
From: A late-stage assembly checkpoint of the human mitochondrial ribosome large subunit
a Consensus map of the mtLSU intermediate viewed from the intersubunit interface. Relevant features are coloured. MRPs are represented in grey and 16S mt-rRNA in light blue. b Clarification of structural heterogeneity of the intersubunit interface by focused classification after masked signal subtraction. States/classes are numbered 1 to 5, corresponding to steps along a putative assembly pathway. Pr: protrusion. Models of relevant regions of states 1 and 4 are presented in Supplementary Fig. 8a. c Comparison between the conformation of H89 in state 1 (surface), state 4 (surface), and the mature mtLSU (ribbon). Rotation of the apex (state 1) and shift in the base (state 4) of H89 are represented by red arrows. Density corresponding to bL36m in each state is shown as surface. The conformation of H89 and density of bL36m in states 2 and 3 are similar to that modelled in state 1, and those in state 5 are represented by state 4. Density of bL36m is presented for states 1–5 in Supplementary Fig. 8b.
