Fig. 7: DmMRM2 is detrimental for the development of D. melanogaster, in particular towards the end stages of pupation.

a Levels of DmMRM2 transcripts in L3 larvae ubiquitously expressing control (n = 3) or DmMRM2 (n = 4) RNAi under the da-GAL4 driver. Values are normalised by the level of αTub84B transcripts. Data are presented as individual datapoints and mean ± SD. Each datapoint was generated from three L3 larvae. Statistical significance was assessed using two-tailed Student’s t test (∗∗: P ≤ 0.01; P = 0.0019). b Dorsal view of whole-body and thorax of control and DmMRM2 knock-down adult flies. c Immunoblot assessment of the steady state levels of MRC subunits over the course of pupation in control (C) and DmMRM2 knock-down (KD) individuals. L3: third instar larvae; P1: day 1 pupae; P2: day 2 pupae; P3: day 3 pupae; P4: day 4 pupae; A1: adults after eclosion. Molecular weights of protein standards are presented in kDa to the right of each blot. Coomassie brilliant blue (CBB) staining is shown as a loading indicator. This experiment was replicated twice with similar results. d Startle-induced negative geotaxis (climbing) assay performed on adult male flies expressing control (n = 70) or DmMRM2 (n = 56) RNAi under the pan-muscular Mef2-GAL4 driver. Data are presented as mean ± SEM. Statistical significance was assessed using the Mann-Whitney test (∗∗∗∗: P ≤ 0.0001; P < 0.00001). Control RNAi: UAS-lacZ RNAi; da-GAL4 (transcript quantification, imaging, immunoblotting), UAS-lacZ RNAi; Mef2-GAL4 (climbing assay). Source data are provided as a Source Data file.