Fig. 3: B-cell subsets display the strongest transcriptional differences between alive and deceased patients on day 7.
From: Cell specific peripheral immune responses predict survival in critical COVID-19 patients
a UMAP embedding plots of B-naive, B-intermediate, B-memory cells, and plasmablasts for the following sample conditions: control, Alive day 7, and Deceased day 7. b UMAP embedding plots of B-cell subsets with imputed CITEseq surface-protein expression for canonical markers. c Hierarchical clustering heatmap of average normalized gene expression for statistically significant differentially expressed genes (adjusted p-value < 0.05 and log2FC > 0.50) between Alive and Deceased day 7 B-naive, B-intermediate, and B-memory cells. d Venn diagram of activation genes from c denoting overlapping signatures across B-cell subsets. e B-cell activation signature z-score for all genes in d overlaid on UMAP embedding plots of B-cells (left) and quantified (right). f Venn diagram of cell-cycle regulation genes from c denoting overlapping signatures across B-cell subsets. g B-cell activation signature z-score for all genes in f overlaid on UMAP embedding plots of B-cells (left) and quantified (right). h Hierarchical clustering heatmap of average normalized gene expression for statistically significant differentially expressed genes (adjusted p-value < 0.05 and log2FC > 0.50) between Alive and Deceased day 7 plasmablasts (left) and antibody processing gene z-scores overlaid on UMAP embedding plots of plasmablasts (middle) and quantified (right). i SARS-CoV-2 IgGII serology at day 7 in critical COVID-19 cohort by outcome. j Interferon signaling gene z-scores overlaid on UMAP embedding of B-cell subsets with quantification in B-naive cells (left) and plasmablasts (right). On all heatmaps blue (low) to red (high) expression. 3261 Control, 5270 Alive day 7, and 2397 Deceased day 7 B-cells were examined across 18 patients. 135 Control, 804 Alive Day 7, and 287 Deceased Day 7 Plasmablasts were examined across 18 patients. Ordinary one-way ANOVA statistical tests were used for each comparison. ** denotes p < 0.01, **** denotes p < 0.0001, and ns denotes not significant.
