Fig. 2: Targeted autophagic degradation of endogenous oncoproteins by AUTOTAC.

a A model illustrating oncoproteins-targeting AUTOTAC. b Chemical structures of oncoprotein-targeting AUTOTAC. c In vitro p62 oligomerization assay in HEK293T cells incubated with PHTPP-1304, VinclozolinM2-2204, or Fumagillin-105. d Western blot (WB) in HEK293T cells treated with PHTPP-1304 at the indicated concentrations (24 h). e Densitometry of (d) (n = 3). f, g WB in ACHN and MCF7 cells, respectively, treated with PHTPP-1304 at the indicated concentrations (24 h). h WB in MCF7 cells treated with PHTPP-1304, PHTPP or YOK-1304 (1 µM, 24 h). i WB in LNCaP cells treated with VinclozolinM2-2204 at the indicated concentrations. j Densitometry of (i) (n = 3). k WB in LNCaP cells treated with VinclozolinM2-2204, Vinclozolin or YOK-2204 (1 µM, 24 h). l WB in HEK293T cells treated with Fumagillin-105, Fumagillin or YTK-105 at the indicated concentrations (24 h). m Densitometry of (l) (n = 3). n WB in U87-MG cells treated with Fumagillin-105 at the indicated concentrations (24 h). o WB in U87-MG cells treated with Fumagilin-105, Fumagilin or YTK-105 (1 µM, 24 h). When indicated, n = 3 biologically independent experiments. Data are presented as mean values ± SD where relevant. Source data are provided with this paper.