Fig. 2: mRNA cap 2′-O-ribose methylation is required for reward learning in Drosophila. | Nature Communications

Fig. 2: mRNA cap 2′-O-ribose methylation is required for reward learning in Drosophila.

From: CMTr cap-adjacent 2′-O-ribose mRNA methyltransferases are required for reward learning and mRNA localization to synapses

Fig. 2

a, b Appetitive memory immediately (a) and 24 h (b) after training of control (white) and CMTr113A (light gray) and CMTrM32 (dark gray) single and double mutant (black) flies shown as mean ± SE. n = 8 for A and n = 6 for B, except CMTr113A/2M32 n = 10, p ≤ 0.006. c Rescue of the learning defect in CMTr113A; CMTr2M32 double mutant flies by genomic fragments (blue) shown as mean ± SE. n = 6, p = 0.002. d, e Rescue of the learning defect in CMTr113A; CMTr2M32 double mutant (black) flies by constitutive (d, n = 8, except UAS-CMTr2 in light gray and MB-GAL4 UASCMTr2 in blue in CMTr113A/2M32 n = 6, dark gray: MB-GAL4) or conditional (e, n = 6, except MBGSG-GAL4 UASCMTr2 in CMTr113A/2M32 n = 8) expression of CMTr2 in mushroom bodies from UAS in the absence (light gray) or presence (dark gray) of RU486 shown as mean ± SE, p ≤ 0.0001. Statistical analysis was done by one-way ANOVA followed by Dunnett’s multiple comparison test. Source data for learning and memory experiments are provided as a Source Data file.

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