Fig. 5: DepMap analysis reveals co-dependency of KIRREL1 and known Hippo pathway genes in CRISPR proliferation screens. | Nature Communications

Fig. 5: DepMap analysis reveals co-dependency of KIRREL1 and known Hippo pathway genes in CRISPR proliferation screens.

From: Cell adhesion molecule KIRREL1 is a feedback regulator of Hippo signaling recruiting SAV1 to cell-cell contact sites

Fig. 5

a Top KIRREL1 correlated genes in Project Achilles. Pearson correlation score on the right indicates the strength of co-dependency between KIRREL1 and indicated genes. b Top SAV1 correlated genes in Project Achilles. c Top NF2 correlated genes in Project Achilles. d Dot plot analysis of cancer cell lines with KIRREL1 mRNA expression (y-axis) plotted against KIRREL1 dependency score (x-axis). High CERES score suggests increased cell proliferation upon gene knockout. e Knockout of KIRREL1 increases expression of YAP target genes in cancer cell lines. Data represent mean ± SD, n = 4, error bars denote the SD between four biological replicates; Unpaired two-tailed t-test was used to determine the statistical significance ***p value < 0.001. f Knockout of KIRREL1 promotes proliferation of cancer cell lines. Cancer cell lines expressing control or KIRREL1 gRNAs were plated in 12-well plates, and cell numbers were quantified at indicated time-points. Data represent mean ± SD, n = 3, error bars denote the SD between three replicates; two-way ANOVA was used without any adjustments to determine the statistical significance. ns—p value > 0.05, *p value < 0.05, **p value < 0.01, ***p value < 0.001. The exact p values are provided as source data. Source data for Fig. 5e, f are provided as Source Data file.

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