Fig. 2: Single-cell RNA-sequencing identifies a unique stroke-associated myeloid cell (SAMC) population.
From: Stroke induces disease-specific myeloid cells in the brain parenchyma and pia
A Merged Uniform Manifold Approximation and Projection (UMAP) plot representing 21 color-coded cell clusters identified in the combined single-cell transcriptomes obtained from brain, dura and pia of sham (ctrl) and MCAO mice (24 h and 72 h post stroke). Cluster names were manually assigned. B Dot plot of selected marker genes characterizing the clusters shown in A. C UMAP plots of single-cell transcriptomes obtained from brain of sham (ctrl) and MCAO mice, 24 h (24 h) and 72 h (72 h) post stroke. D Bi-directional histograms depicting differences in relative cluster abundance between MCAO (24 h and 72 h post ischemia) and sham (ctrl) samples as shown in C. Differences are calculated as: percentage of all cells in the respective stroke sample minus percentage of all cells in the control sample. Cluster annotations and cluster coloring are consistent between all panels. Abbreviation key: Bc B cell, CAM central nervous system-associated macrophages, CNS central nervous system, gdTc γδ T cells, granulo granulocytes, ILC2 innate lymphoid cells type 2; Macro macrophage, Mast mast cells, mDC myeloid dendritic cell, Micro microglia, NK natural killer cells, proli_cells proliferating cells, SAMC stroke-associated myeloid cells, stress_Micro stressed Microglia; stress_Myeloid, stressed Myeloid cells; Tc, T cells. Source data for D are provided as a Source Data file.
