Fig. 3: Identification of two calcium ions that mediate the binding of Cy.004 to CyRPA.
From: Heterotypic interactions drive antibody synergy against a malaria vaccine candidate
A Cy.004 Fab fragment (pink) bound to CyRPA (blue) with the two calcium ions shown as orange spheres. The inset shows a close up of the calcium-binding site with the coordinating residues labelled. B The absolute abundance of different metal ions within the Cy.004:CyRPA complex as determined by microPIXE measurements. Bars indicate the mean and standard deviation across n = 3 replicate measurements. C SPR sensorgrams of Cy.004 (immobilized) binding to CyRPA in TBS with or without 1 mM MgCl2 or 1 mM CaCl2. Each curve shows the outcome of a two-fold dilution series of CyRPA from 100 to 3.1 nM. The black line indicates measured response while the red line indicates 1:1 curve fit.
