Fig. 7: Recovery of gene expression (Microarray) in Mig-6^d/d mice by Erbb2 double ablation.

a Clustering analysis of Mig-6 dependent regulated genes in uteri of control, Mig-6^d/d, and Mig-6^d/dErbb2^d/d mice at GD 3.5. The extent of gene expression changes is represented by a green-red color scale (green: low expression and red: high expression). b RT-qPCR analysis of transcript amounts of Mig-6 dependent regulated genes, Mcm2 (* p = 0.0108 and * p = 0.0133), Mcm3 (* p = 0.0210 and ** p = 0.0075), Mcm4 (* p = 0.0459 and * p = 0.0266), Mcm5 (* p = 0.0286 and * p = 0.0176), Mcm6 (*** p = 0.0005 and *** p < 0.0001), Klf15 (* p = 0.0219 and ** p = 0.0022), Klf4 (* p = 0.0232 and ** p = 0.0085), Hells (** p = 0.0029 and ** p = 0.0047), Fen1 (** p = 0.0074 and ** p = 0.0059), Pcna (* p = 0.0108 and * p = 0.0121), Scl25a13 (* p = 0.0104 and ** p = 0.0029), Ccnd1 (** p = 0.0056 and ** p = 0.0072), Tgfa (* p = 0.0364 and ** p = 0.0016), and Cdkl1 (* p = 0.0189 and ** p = 0.0041) in uteri of control, Mig-6^d/d, and Mig-6^d/dErbb2^d/d mice at GD 3.5 (n = 5 per each genotype of upper graph and n = 6 per each genotype in bottom graph). Data are represented as mean ± SEM and analyzed by Ordinary one-way ANOVA test. c Immunohistochemistry analysis of MCM2 (** p = 0.0011 and *** p = 0.0001) and MCM6 (* p = 0.0227 and * p = 0.00184) in the uterus of control, Mig-6^d/d, and Mig-6^d/dErbb2^d/d mice at GD 3.5 (n = 5 per each genotype). Data are represented as mean ± SEM and analyzed by Ordinary one-way ANOVA test. Scale bars: 50 μm. d Immunohistochemistry analysis of KLF4 (*** p < 0.0001) and KLF15 (*** p = 0.0008 and *** p = 0.0002) in the uterus of control, Mig-6^d/d, and Mig-6^d/dErbb2^d/d mice at GD 3.5 (n = 5 per each genotype). Data are represented as mean ± SEM and analyzed by Ordinary one-way ANOVA test. Scale bars: 25 μm. Three independent experiments were performed for (b–d) with similar results. Source data are provided in the Source Data file.