Fig. 4: CPEB1 regulates the translational landscape for SC activation.

a–e CPEB1-dependent proteome analysis. a Schematic illustration showing the workflow of proteomic analysis on CPEB1 knocked down SCs. b Hierarchical clustering of the protein expression (in the number of spectra) of SCs after CPEB1 knockdown. c Heatmap of representative protein expression for cell cycle genes. d Quantification of differentially expressed proteins after CPEB1 knockdown. e Functional enrichment analysis of CPEB1-dependent proteins using Reactome¹⁰⁰. (n = 2 independent experiments). In Reactome the method used to calculate the statistical significance is the binomial test. f, g Heatmaps of representative protein expression involved in MAPK6/MAPK4 signaling, mRNA stability regulation, myogenesis, and translation identified in e. h Venn diagram of CPEB1-associated genes, upregulated proteins in cASCs, and CPEB1-dependent proteins. i Bar graph showing percentage of CPE-containing genes of the 67 proteins in the intersect of h. j Functional enrichment analysis of the 67 proteins in Biological Process (BP) pathways by g: Profiler⁹⁹. The g:Profiler uses a hypergeometric test to measure the significance of functional term in the input gene list. k Heatmap of the protein expression of the 67 proteins in the intersect of h.