Fig. 2: Defining the molecular signature of FL-derived HSCs with the highest engraftment potential.

a Engraftment data demonstrating superior engraftment of the GPI-80⁺ fraction 16 weeks post transplantation. Five NSG mice were transplanted per condition with 10,000 cells each (4000 cells for the GPI-80+ condition) and engraftment was assessed by determining the ratio (%) of peripheral blood cells expressing human CD45 versus total CD45 expressing cells. The mean is shown with error bars representing the standard error of the mean. One-way analysis of variance (ANOVA) was performed with Tukey test to account for multiple comparisons and obtain adjusted p-values: GPI-80⁺ vs. CD34⁺bulk p = 0.002, GPI-80⁺ vs. CD34^-GYPA^- p = 0.001 (**p < 0.01). Source data are provided as a Source Data file. b Violin plots of differentially expressed genes between HSCs/MPPs in the GPI-80⁺ and CD34⁺ bulk fractions (left) and bar plots illustrating the percentage of cells expressing these genes per fraction (right). c UMAP representation showing the clusters identified in the CD34⁺ HSC/MPP population. d UMAP representation of the HSCs/MPPs corresponding to the CD34⁺ bulk sample, retaining the cluster definitions established in panel c. e UMAP representation of the HSCs/MPPs corresponding to the GPI-80⁺ sample, retaining the cluster definitions established in panel c. Additional labeling with one of the top most enriched genes is shown for each cluster (see Table S4). f Cluster dynamics upon GPI-80-enrichment showing the proportion of each cluster before (CD34⁺bulk) and after (GPI-80⁺) functional enrichment in a stacked bar plot. Percentages of cells in each cluster are shown on the left. g UMAP overlays illustrating mRNA expression patterns of genes enriched in different GPI-80⁺ clusters. The scale bar shows scaled expression from 0-Max. h Regulon specificity score (RSS) plot showing the regulons with the highest specificity for a given cluster within the GPI-80⁺ fraction. The scale bar represents z-scores and dot sizes reflect the RSS for each regulon. i Overlay of the expression patterns of cluster-specific regulons onto the GPI-80⁺ UMAP.