Fig. 3: Integration of mRNA and antibody-derived tag (ADT) data identifies CD201 as a marker enriching for highly functional HSCs.

a Comparison of mRNA and ADT expression for a selection of HSC markers in the CD34⁺bulk sample. b Dot plot illustrating the distribution of antibody-derived tag (ADT) expression for a selection of HSC markers in the GPI-80⁺ fraction versus the CD34⁺ bulk fraction. Expression is represented as scaled average expression and the scale is kept constant between the CD34⁺ bulk and GPI-80⁺ fraction for each marker to allow for comparison of relative intensities. “Percent expressed” represents the fraction of cells having non-zero expression values for each cell cluster in the two samples. c–e Comparison of the ability to enrich for cluster 0 across the different cell-surface markers included in our ADT panel for the CD34⁺bulk fraction (c), GPI-80⁺ fraction (d), and combined analysis of all 4 originally sorted samples (e). The proportion of cells representing cluster 0 (%) is shown on the y-axis, considering cells enriched for each marker (top 5%) on the x-axis. f, g Transcriptomic cluster composition (%) within the CD34⁺ bulk fraction upon stepwise enrichment for cell-surface marker expression of CD201 (f) and CD164 (g). The x-axis is divided in segments showing the indicated top x% of expression for each marker. h Engraftment data demonstrating superior engraftment of the CD34⁺CD201⁺ fraction compared to the CD34⁺GPI-80⁺ fraction 20 weeks post transplantation. Six NSG mice were transplanted per condition with 2500 cells each and engraftment was assessed by determining the percentage (%) of peripheral blood cells expressing human CD45. The mean is shown for each condition with error bars representing the standard error of the mean. One-way analysis of variance (ANOVA) was performed with Tukey test to account for multiple comparisons and obtain adjusted p-values: *p = 0.0179, ****p < 0.0001. Source data are provided as a Source Data file. Longitudinal analysis and demonstration of multilineage repopulation can be found in Supplementary Fig. 4f–h.