Fig. 1: Expression pattern of wg, apAB and vg in thoracic segments of Gryllus.

a Schematics illustrate lateral views of Gryllus embryos in each stage. Magenta rectangle indicates the region shown in b–j. Dorsolateral regions of thoracic segments stained for wg (b–d), apAB (e–g) and vg (h–j) with in situ hybridization in stage 6 to early stage 7 (b, e, h), late stage 7 (c, f, i) and early stage 9 (d, g, j). Each image shows a representative result of at least three independent experiments with similar results. k Summary of gene expression pattern in meso- and metathoracic segments. Red and blue lines indicate tergal and pleural regions, respectively. l–n vg reporter gene signals in dorsolateral regions of thoracic segments in late embryos. o–r and o’–r’ vg reporter gene signals in postembryonic stages. Dorsolateral region (o, o’) and thoracic musculature in median section (p, p’) of sixth instar nymph, and forewing (q, q’) and thoracic musculature in median section (r, r’) of adult. Crickets before cuticle coloration are shown for both nymph and adult. Red and blue arrowheads indicate expression in tergal and pleural regions, respectively. Scale bars are 100 µm in b–j, 250 µm in l–n and 3 mm in o–r, respectively.