Fig. 4: Wnt, Ft-Ds and Hippo signaling are required for the postembryonic wing growth in Gryllus. | Nature Communications

Fig. 4: Wnt, Ft-Ds and Hippo signaling are required for the postembryonic wing growth in Gryllus.

From: A hemimetabolous wing development suggests the wing origin from lateral tergum of a wingless ancestor

Fig. 4

a Schematics illustrates tergal regions used for the transcriptome comparison. Note that only T2 and T3 segments were used for this analysis. b A volcano plot shows differential expression of transcripts between central and lateral regions in T2 and T3 terga. Data after log-fold change shrinkage with the apeglm method is visualized. Significantly differentially expressed transcripts (adjusted P < 10e−5, fold change >1.5) are in red. A Wald test was used for significance testing and P values were adjusted using the Benjamini and Hocheberg method according to DESeq2 software. Some representative transcripts that are highly expressed in the lateral region are labeled. c Heatmap indicating expression level of 33 candidate genes in each sample. Color code indicates z-score normalized transcripts per million (TPM). d–i Adult phenotypes after nymphal RNAi treatment. Fore- and hindwings are shaded in magenta and cyan, respectively. Scale bars are 2 mm.

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