Fig. 3: GIT1 directly binds to the Notch intracellular domain.
From: GIT1 protects against breast cancer growth through negative regulation of Notch
a, b Co-IP of GIT1 with Notch1 from lysates of the indicated mouse organs (a) and human breast cell lines (b). c Co-IP of the Notch1-2 ICDs with GIT1 from lysates of the MDA-MB-231 cells treated as indicated. d, Schematic diagrams of recombinant fusion protein fragments. e, f GIT1 pulled down by GST Notch fragments from MDA-MB-231 cell lysates. g Pulldown of purified GIT1-His by purified GST-N1ICD/N. CB, Coomassie Blue. Images from representative blots; the experiment was repeated n = 3 times with similar results. *, indicates recombinant GST-fused peptides. Source data are provided as a Source Data file.
