Table 1 Summary of structural and refinement statistics.
From: Cryo-EM structure of translesion DNA synthesis polymerase ζ with a base pair mismatch
Polζ-mismatch (A:C)-dCTP | |
|---|---|
Data collection and processing | |
Magnification | ×105,000 |
Voltage (kV) | 300 |
Pixel size (Å/pixel) | 1.096 |
Electron dose (e−/ Å2) | 64.82 |
Defocus range (µm) | 0.5–2.5 |
Number of micrographs | 2279 |
Number of particles | 120,985 |
Symmetry imposed | C1 |
Nominal map resolution (Å) | 3.05 |
FSC threshold | 0.143 |
Map sharpening B-factor (Å2) | −102 |
Refinement (phenix) | |
Initial model used (PDB code) | 6V93 |
Model resolution (Å) | 3.4 |
FSC threshold | 0.5 |
Model composition | |
Non-hydrogen atoms | 17,099 |
Protein residues | 2180 |
DNA/other | 24/3 |
B-factors (Å2) | |
Protein | 79.09 |
DNA/other | 118.32/92.67 |
R.M.S. deviations | |
Bond length (Å) | 0.002 |
Bond angles (°) | 0.495 |
Validation | |
Molprobity score | 1.74 |
Clashscore | 5.98 |
Rotamer outliers (%) | 0.00 |
Cβ outliers (%) | 0.00 |
Ramachandran statistics (%) | |
Favored | 93.87 |
Allowed | 6.08 |
Outliers | 0.05 |
