Fig. 1: Uterine-specific deletion of Men1 leads to female subfertility. | Nature Communications

Fig. 1: Uterine-specific deletion of Men1 leads to female subfertility.

From: Menin directs regionalized decidual transformation through epigenetically setting PTX3 to balance FGF and BMP signaling

Fig. 1

a In situ hybridization of Men1 in WT uteri on days 1, 4, 5, 6, and 8 of pregnancy. Scale bars: 500 μm. b Immunohistochemical analysis of Menin in day 4 uteri and days 5, 6, and 8 implantation sites. Le luminal epithelium, Ge glandular epithelium, S stroma, Bl blastocyst, Em embryo. Scale bar: 200 μm. c Quantitative real-time PCR analysis of Men1 mRNA levels in Men1f/f and Men1d/d implantation sites on day 6. The values are normalized to Gapdh and indicated as the mean ± SEM (n = 8 biologically independent samples). Two-tailed unpaired Student’s t-test, ****p = 4.26e-7. d Immunoblotting analysis of Menin protein in Men1f/f and Men1d/d implantation sites on day 6. Β-Actin was used as a loading control. e Immunohistochemical analysis of Menin in Men1f/f and Men1d/d implantation sites on day 6. S stroma, Em embryo. Scale bar: 200 μm. f Pregnancy rates in Men1f/f and Men1d/d female mice. The number within brackets indicates females with pups over total number of plug-positive females. g Average litter sizes in Men1f/f (n = 20 animals) and Men1d/d (n = 5 animals) mice. Data represent the mean ± SEM. Two-tailed unpaired Student’s t-test, ****p = 4.31e-8.

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