Fig. 6: H3K4me3 levels are decreased in a limited number of genes downregulated in Men1-deficient uteri.

a Genomic distribution of Menin and H3K4me3 peaks in decidual tissues on day 8. b Menin and H3K4me3 ChIP-seq signal density at TSS. TSS, transcriptional start site. c Correlation between Menin and H3K4me3 ChIP-seq signal in gene promoters. R² = 0.5888291. d Change of H3K4me3 reads the number at gene promoters between Men1^f/f and Men1^d/d decidual tissues on day 8. ****p = 2.2e-16. The boxplots where midlines indicate medians, boxes indicate the interquartile range and whiskers indicate minimum/maximum range, and the statistical significance was calculated by the Wilcoxon test. e Genome browser view of normalized Menin and H3K4me3 ChIP-seq signals and RNA-seq tracks for known target genes in Men1^f/f and Men1^d/d decidual tissues on day 8. f Coverage profiles for Menin and H3K4me3. Heatmap of peaks with decreased H3K4me3 following Men1 deletion centered at TSS. TSS transcriptional start site. g Venn diagram showing the overlap between genes with reduction H3K4me3 in promoters by Men1 loss (n = 6,469), genes with promoter bound by Menin (n = 7364) and genes differentially expressed upon Men1 loss (n = 1659). h Heatmap of top-ranked genes that are most likely to be the targets of Menin-H3K4me3. RPKM of each gene from different samples was normalized to Z-score. i Genome browser view of normalized ChIP-seq signals and RNA-seq tracks for Menin-H3K4me3 target genes in Men1^f/f and Men1^d/d decidual tissues.