Fig. 4: Role of the different enhancers composing the SE. | Nature Communications

Fig. 4: Role of the different enhancers composing the SE.

From: A fast Myosin super enhancer dictates muscle fiber phenotype through competitive interactions with Myosin genes

Fig. 4

A Schematic representation of the snATAC-seq peaks along the 42 kb SE and the enhancers A and B deleted by CRISPR/Cas9 editing. B Immunostaining against fast MYH2, MYH4, and slow MYH7 on adult leg sections of 2–3-month-old mouse female deleted for enhancer A or B. C Same as B, zoom in Tibialis posterior and FHL muscle of WT and EnhA−/− mutant. D Immunostaining against fast MYH1 in Tibialis posterior and FHL muscle of WT and EnhA−/− mutant. The absence of EnhA induced an increased number of MYH1 positive fibers. E Quantification of fMyh mRNA and of Linc-Myh in adult TA of control and EnhA and EnhB mutant by RT-qPCR experiments. For E, n = 3. Numerical data are presented as mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars: 100 μm for B–D. Significance of difference, for E: one-way ANOVA with multiple comparisons. Source data are provided as a Source Data file.

Back to article page