Fig. 1: Gm9999 encodes Kastor and Polluks. | Nature Communications

Fig. 1: Gm9999 encodes Kastor and Polluks.

From: Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis

Fig. 1

a PhyloCSF scores and expression levels of 996 testis-specific lncRNAs of mouse in the NCBI database. RPKM, reads per kilobase of transcript per million mapped reads. b Schematic representation of the Gm9999 locus encoding Kastor and Polluks. Two mRNA isoforms with different transcription start sites are transcribed from the Gm9999 locus on mouse chromosome (Chr) 7 and encode Kastor or Polluks. CDS, coding sequence; aa, amino acids. c Conservation of Kastor, Polluks, VDAC1, VDAC2, and VDAC3 among species analyzed by tBlastN. d RT-PCR analysis of the indicated mRNAs in adult mouse tissues. e In situ hybridization analysis of adult mouse testicular sections with riboprobes for Kastor or Polluks mRNAs. Nuclei were stained with nuclear fast red. Scale bars, 50 μm. f, g Immunoblot analysis of the testis (f) and spermatozoa collected from the cauda epididymis (g) of KastorFLAG/+ and PolluksFLAG/+ adult mice was performed with antibodies to FLAG and to α-tubulin (loading control). h Immunoblot analysis of adult mouse tissues with antibodies to Kastor, to Polluks, or to HSP90 (loading control, short and long exposures for which are shown). Source data are provided as a Source data file.

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