Fig. 4: Both Kastor and Polluks bind to VDAC. | Nature Communications

Fig. 4: Both Kastor and Polluks bind to VDAC.

From: Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis

Fig. 4

a Lysates either of HEK293T cells transiently expressing Kastor-(C)FLAG or Polluks-(C)FLAG or of the testis of adult KastorFLAG/+ or PolluksFLAG/+ mice were subjected to immunoprecipitation with antibodies to FLAG, and the resulting precipitates were subjected to SDS–polyacrylamide gel electrophoresis (PAGE) and silver staining. b Intensity-based absolute quantification (iBAQ) of VDAC isoforms in immunoprecipitates prepared as in (a). c, d Immunoblot analysis with the indicated antibodies of immunoprecipitates (IP) prepared from HEK293T cells (c) or from mouse testis (d) as in (a). The original cell and tissue lysates (Input) were also analyzed. e In vitro binding assay for purified Kastor-(C)FLAG or Polluks-(C)FLAG with VDAC3 or ARI (negative control). FLAG immunoprecipitates isolated from the binding reaction mixtures were subjected to SDS-PAGE followed by staining with Coomassie brilliant blue. Source data are provided as a Source data file.

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