Fig. 8: Lack of effect of VDAC3 or Kastor/Polluks ablation on mitochondrial metabolism and mPTP opening.
a, b Metabolome analysis of spermatozoa collected from the cauda epididymis of adult dKO (a) or VDAC3 KO (b) mice. Cit, citric acid; cis-Aco, cis-aconitic acid; IsoCit, isocitric acid; 2KG, 2-ketoglutaric acid; Mal, malic acid. c Mitochondrial membrane potential of spermatozoa collected from the cauda epididymis of adult mice of the indicated genotypes. Carbony cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) was used as a control. d–g Oxygen consumption rate (OCR) traces for spermatozoa collected from the cauda epididymis of adult dKO (d) or VDAC3 KO (e) mice, as well as quantification of basal respiration, ATP production, maximal respiration, and spare capacity for the dKO (f) and VDAC3 KO (g) spermatozoa. h, i Analysis of mPTP opening in spermatozoa collected from the cauda epididymis of adult mice. Spermatozoa of WT mice were treated with the indicated agents (h), and those of dKO or VDAC3 KO mice were treated with calcein-AM and CoCl2 (i). j Calcium ion concentration in mitochondria of spermatozoa collected from the cauda epididymis of adult mice of the indicated genotypes. k ROS formation in spermatozoa collected from the cauda epididymis of adult mice of the indicated genotypes. l Quantitative analysis of the number of TUNEL-positive cells per seminiferous tubule in testicular sections of adult mice of the indicated genotypes. m Quantitative analysis of the percentage of spermatozoa isolated from the cauda epididymas of adult mice that were positive for propidium iodide (PI) staining. All quantitative data are means ± s.d. for the indicated numbers of mice. *P < 0.05, **P < 0.01, ***P < 0.001; NS, not significant (paired two-tailed Student’s t test, unpaired two-tailed Student’s t test, one-way ANOVA, or two-way ANOVA). Source data are provided as a Source data file.
