Fig. 2: Mild fasting metabolic defects in NAMPTLKO mice.
A Heat-Zeitgeber Time tracing in WT and NAMPTLKO littermate mice during light and dark cycles during fasting. Mean heat production in WT and NAMPTLKO mice during light and dark cycle fed and early (Fasting I, 1- 24 h) and prolonged (Fasting II, 24–48 h) fasting periods is graphed below. n = 3 WT, 4 NAMPTLKO mice. B RER-Zeitgeber time tracing in WT and NAMPTLKO littermate mice during light and dark cycles during ad-lib fed (first 24 h) and fasting periods. Mean RER in WT and NAMPTLKO mice during light and dark cycle fed and fasting periods is graphed below. n = 3 WT, 4 NAMPTLKO mice. Error bars in (A–B) (bottom panels) represent SEM. Circles, WT mice. Squares, NAMPTLKO mice for (A and B). C Liver triglycerides (TG), total cholesterol and non-esterified fatty acid (FFA) quantification following 48 h fasting in WT and NAMPTLKO mice. n = 4 WT Fed, 6 WT Fasting; 4 NAMPTLKO Fasting. D qPCR quantification of liver FGF21, NAMPT in fed and 48 h-fasting WT and NAMPTLKO mice. n = 4 WT Fed, 6 WT Fasting; 4 NAMPTLKO Fasting. Error bars in (C and D). represent SEM. Black circles, Fed WT, Red circles, fasting WT, Blue circles, Fasting NAMPTLKO mice. E Venn diagram representing significantly altered genes (P < 0.05) in random-fed vs. 12 h fasting WT and NAMPTLKO mice, quantified by unbiased transcriptomics. n = 2 WT Fed, 4 WT Fasting; 5 NAMPTLKO Fasting. F Bar plot demonstrating −log(FC) for significantly down-regulated gene ontology (GO) pathways revealed when comparing WT vs. NAMPTLKO livers (P < 0.05). n = 4 WT Fed, 6 WT Fasting; 4 NAMPTLKO Fasting. Statistical tests: (A) (upper panel), ANCOVA. A (lower panels), (B–D), two-tailed T-test, Bonferroni–Dunn post hoc correction. E, F EdgeR Exact, Benjamini–Hochberg post hoc correction.
